Protease Inhibitor:Article Title: CDKL5 deficiency in adult glutamatergic neurons alters synaptic activity and causes spontaneous seizures via TrkB signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Corn oil ABCONE Cat# C67366 Pentobarbital sodium Sigma Cat# P3761 Ethanol absolute Sinoreagent Cat# 10009228 Triton X-100 Sigma Cat# 9036-19-5 PBS Sigma Cat# P3744 Xylene Sigma Cat# 1330-20-7 Paraformaldehyde Sigma Cat# 30525-89-4 DAPI Sigma Cat# 28718-90-3 Chloroform Sigma Cat# 487163 Tissue-TekTM O.C.T. .. Compound SAKURA Cat# 4583 Fluoromount-G Solarbio Cat# S2100 NaF ProSpec Cat# hor-248 Na3VO4 Sigma Cat# 590088 EDTA Invitrogen Cat# 15576028 EGTA Sigma Cat# E3889 PMSF Acmec Cat# AP0100 Tris base Sigma Cat# 93350 Sucrose Sigma Cat# S0389 PhosSTOP Roche Cat# 4906837001 Protease inhibitor cocktail Acmec Cat# AC13365 NaCl Biomed Cat# SH5001-01 NP-40 Thermo Cat# 85124 SDS Beyotime Cat# ST626 Sodium deoxycholate Sigma Cat# 30968 Fat-free milk Yili Cat# 100006817794 Tween 20 Adamas life Cat# 89190D ReBlot Plus Strong Antibody Stripping Solution, 10x Sigma Cat# 2504 Chloral hydrate Sigma Cat# C8383 Trizol Invitrogen Cat# 15596026 NMDG Sigma Cat# M2004 KCl Beyotime Cat# ST345 NaH2PO4 Sigma Cat# 229903 NaHCO3 Sigma Cat# W700433 HEPES Sigma Cat# H23830 Glucose Sigma Cat# 50-99-7 Sodium pyruvate Sigma Cat# 105477 Sodium ascorbate Sigma Cat# Y0000039 Thiourea Sigma Cat# T8656 N-Acetyl-L-Cysteine Sigma Cat# PHR1098 MgSO4 NEB Cat# B1003S CaCl2 Sigma Cat# 1086436 MgCl2 Beyotime Cat# R0058 CsMeSO3 Sigma Cat# 184079 QX-314 Absin Cat# abs42026129 Mg-ATP Bioss Cat# C5068 (Continued on next page) 12 Cell Reports 42, 113202, October 31, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Na-GTP Sigma Cat# 51120 Critical commercial assays BCA protein assay kit Tiangen Cat# PA115 FD Rapid GolgiTM Kit FD NeuroTechnologies Cat# PK401 iScript cDNA Synthesis Kit Bio-Rad Cat# 1708891 LightCycler RNA Master SYBR Green I kit Roche Cat# 03064760001 TUNEL Apoptosis Assay Kit Beyotime Cat# C1098 Experimental models: Organisms/strains Cdkl5flox/flox mice Wang et al.8 Cat# JAX 030523 Camk2aCreERT2 mice Erdmann et al.46 N/A TrkBflox/flox mice Grishanin et al.47 Cat# MMRRC 033048-UCD Oligonucleotides Gapdhforward:CCCCAATGTATCCGTTGTG Chen et al.48 N/A Gapdh-reverse: CTCAGTGTAGCCCAGGATGC Chen et al.48 N/A Bdnf-forward: AGGTCTGACGACGACATCACT PrimerBank49–51 PrimerBank ID 114326460c2 Bdnf-reverse: CTTCGTTGGGCCGAACCTT PrimerBank49–51 PrimerBank ID 114326460c2 Software and algorithms Spike2 software (CED Ltd., Micro1401mkII) CED Ltd https://ced.co.uk/products/spkovin ImageJ (Fiji) Software Schneider et al.52 https://imagej.net/software/fiji/ GraphPad Prism 6 GraphPad Software https://www.graphpad.com/features Others EEG and EMG amplifiers A-M SYSTEMS Cat# 1800 Stereotaxic apparatus Harvard apparatus Cat# 75-1828 Video recording GUCEE Cat# HD98 Centrifugal machine Eppendorf Cat# 5720110011 Enhanced chemiluminescence (ECL) Analytik Jena AG Cat# 849-97-0930 Insulated silver wires Coonerwire Cat# AS633 Frozen ball mill Shanghai Jingxin Cat# JXFSTPRP Vertical Electrophoresis Cell Bio-Rad Cat# 1658001FC PVDF membrane Millipore Cat# IPVH00010 LightCycler 480 Real-Time PCR System Roche Cat# 05015243001 Cryostat Leica CM3050 VT 1200S vibratome Leica Cat# 14048142066 MultiClamp 700B amplifier Axon Instruments Cat# 1-CV-7B Digidata 1440A Axon Instruments Cat# 1-2500-0173 D FN1 microscope Nikon FN1 Borosilicate glass capillaries wpi-europe Cat# B100-20-10 Horizontal glass electrode puller Sutter Cat# P-97 Stimulation electrode FHC Cat# 55-00-13 Confocal microscope Nikon A1 Slide Scanner Olympus VS200
Fat:Article Title: CDKL5 deficiency in adult glutamatergic neurons alters synaptic activity and causes spontaneous seizures via TrkB signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Corn oil ABCONE Cat# C67366 Pentobarbital sodium Sigma Cat# P3761 Ethanol absolute Sinoreagent Cat# 10009228 Triton X-100 Sigma Cat# 9036-19-5 PBS Sigma Cat# P3744 Xylene Sigma Cat# 1330-20-7 Paraformaldehyde Sigma Cat# 30525-89-4 DAPI Sigma Cat# 28718-90-3 Chloroform Sigma Cat# 487163 Tissue-TekTM O.C.T. .. Compound SAKURA Cat# 4583 Fluoromount-G Solarbio Cat# S2100 NaF ProSpec Cat# hor-248 Na3VO4 Sigma Cat# 590088 EDTA Invitrogen Cat# 15576028 EGTA Sigma Cat# E3889 PMSF Acmec Cat# AP0100 Tris base Sigma Cat# 93350 Sucrose Sigma Cat# S0389 PhosSTOP Roche Cat# 4906837001 Protease inhibitor cocktail Acmec Cat# AC13365 NaCl Biomed Cat# SH5001-01 NP-40 Thermo Cat# 85124 SDS Beyotime Cat# ST626 Sodium deoxycholate Sigma Cat# 30968 Fat-free milk Yili Cat# 100006817794 Tween 20 Adamas life Cat# 89190D ReBlot Plus Strong Antibody Stripping Solution, 10x Sigma Cat# 2504 Chloral hydrate Sigma Cat# C8383 Trizol Invitrogen Cat# 15596026 NMDG Sigma Cat# M2004 KCl Beyotime Cat# ST345 NaH2PO4 Sigma Cat# 229903 NaHCO3 Sigma Cat# W700433 HEPES Sigma Cat# H23830 Glucose Sigma Cat# 50-99-7 Sodium pyruvate Sigma Cat# 105477 Sodium ascorbate Sigma Cat# Y0000039 Thiourea Sigma Cat# T8656 N-Acetyl-L-Cysteine Sigma Cat# PHR1098 MgSO4 NEB Cat# B1003S CaCl2 Sigma Cat# 1086436 MgCl2 Beyotime Cat# R0058 CsMeSO3 Sigma Cat# 184079 QX-314 Absin Cat# abs42026129 Mg-ATP Bioss Cat# C5068 (Continued on next page) 12 Cell Reports 42, 113202, October 31, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Na-GTP Sigma Cat# 51120 Critical commercial assays BCA protein assay kit Tiangen Cat# PA115 FD Rapid GolgiTM Kit FD NeuroTechnologies Cat# PK401 iScript cDNA Synthesis Kit Bio-Rad Cat# 1708891 LightCycler RNA Master SYBR Green I kit Roche Cat# 03064760001 TUNEL Apoptosis Assay Kit Beyotime Cat# C1098 Experimental models: Organisms/strains Cdkl5flox/flox mice Wang et al.8 Cat# JAX 030523 Camk2aCreERT2 mice Erdmann et al.46 N/A TrkBflox/flox mice Grishanin et al.47 Cat# MMRRC 033048-UCD Oligonucleotides Gapdhforward:CCCCAATGTATCCGTTGTG Chen et al.48 N/A Gapdh-reverse: CTCAGTGTAGCCCAGGATGC Chen et al.48 N/A Bdnf-forward: AGGTCTGACGACGACATCACT PrimerBank49–51 PrimerBank ID 114326460c2 Bdnf-reverse: CTTCGTTGGGCCGAACCTT PrimerBank49–51 PrimerBank ID 114326460c2 Software and algorithms Spike2 software (CED Ltd., Micro1401mkII) CED Ltd https://ced.co.uk/products/spkovin ImageJ (Fiji) Software Schneider et al.52 https://imagej.net/software/fiji/ GraphPad Prism 6 GraphPad Software https://www.graphpad.com/features Others EEG and EMG amplifiers A-M SYSTEMS Cat# 1800 Stereotaxic apparatus Harvard apparatus Cat# 75-1828 Video recording GUCEE Cat# HD98 Centrifugal machine Eppendorf Cat# 5720110011 Enhanced chemiluminescence (ECL) Analytik Jena AG Cat# 849-97-0930 Insulated silver wires Coonerwire Cat# AS633 Frozen ball mill Shanghai Jingxin Cat# JXFSTPRP Vertical Electrophoresis Cell Bio-Rad Cat# 1658001FC PVDF membrane Millipore Cat# IPVH00010 LightCycler 480 Real-Time PCR System Roche Cat# 05015243001 Cryostat Leica CM3050 VT 1200S vibratome Leica Cat# 14048142066 MultiClamp 700B amplifier Axon Instruments Cat# 1-CV-7B Digidata 1440A Axon Instruments Cat# 1-2500-0173 D FN1 microscope Nikon FN1 Borosilicate glass capillaries wpi-europe Cat# B100-20-10 Horizontal glass electrode puller Sutter Cat# P-97 Stimulation electrode FHC Cat# 55-00-13 Confocal microscope Nikon A1 Slide Scanner Olympus VS200
Stripping Membranes:Article Title: CDKL5 deficiency in adult glutamatergic neurons alters synaptic activity and causes spontaneous seizures via TrkB signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Corn oil ABCONE Cat# C67366 Pentobarbital sodium Sigma Cat# P3761 Ethanol absolute Sinoreagent Cat# 10009228 Triton X-100 Sigma Cat# 9036-19-5 PBS Sigma Cat# P3744 Xylene Sigma Cat# 1330-20-7 Paraformaldehyde Sigma Cat# 30525-89-4 DAPI Sigma Cat# 28718-90-3 Chloroform Sigma Cat# 487163 Tissue-TekTM O.C.T. .. Compound SAKURA Cat# 4583 Fluoromount-G Solarbio Cat# S2100 NaF ProSpec Cat# hor-248 Na3VO4 Sigma Cat# 590088 EDTA Invitrogen Cat# 15576028 EGTA Sigma Cat# E3889 PMSF Acmec Cat# AP0100 Tris base Sigma Cat# 93350 Sucrose Sigma Cat# S0389 PhosSTOP Roche Cat# 4906837001 Protease inhibitor cocktail Acmec Cat# AC13365 NaCl Biomed Cat# SH5001-01 NP-40 Thermo Cat# 85124 SDS Beyotime Cat# ST626 Sodium deoxycholate Sigma Cat# 30968 Fat-free milk Yili Cat# 100006817794 Tween 20 Adamas life Cat# 89190D ReBlot Plus Strong Antibody Stripping Solution, 10x Sigma Cat# 2504 Chloral hydrate Sigma Cat# C8383 Trizol Invitrogen Cat# 15596026 NMDG Sigma Cat# M2004 KCl Beyotime Cat# ST345 NaH2PO4 Sigma Cat# 229903 NaHCO3 Sigma Cat# W700433 HEPES Sigma Cat# H23830 Glucose Sigma Cat# 50-99-7 Sodium pyruvate Sigma Cat# 105477 Sodium ascorbate Sigma Cat# Y0000039 Thiourea Sigma Cat# T8656 N-Acetyl-L-Cysteine Sigma Cat# PHR1098 MgSO4 NEB Cat# B1003S CaCl2 Sigma Cat# 1086436 MgCl2 Beyotime Cat# R0058 CsMeSO3 Sigma Cat# 184079 QX-314 Absin Cat# abs42026129 Mg-ATP Bioss Cat# C5068 (Continued on next page) 12 Cell Reports 42, 113202, October 31, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Na-GTP Sigma Cat# 51120 Critical commercial assays BCA protein assay kit Tiangen Cat# PA115 FD Rapid GolgiTM Kit FD NeuroTechnologies Cat# PK401 iScript cDNA Synthesis Kit Bio-Rad Cat# 1708891 LightCycler RNA Master SYBR Green I kit Roche Cat# 03064760001 TUNEL Apoptosis Assay Kit Beyotime Cat# C1098 Experimental models: Organisms/strains Cdkl5flox/flox mice Wang et al.8 Cat# JAX 030523 Camk2aCreERT2 mice Erdmann et al.46 N/A TrkBflox/flox mice Grishanin et al.47 Cat# MMRRC 033048-UCD Oligonucleotides Gapdhforward:CCCCAATGTATCCGTTGTG Chen et al.48 N/A Gapdh-reverse: CTCAGTGTAGCCCAGGATGC Chen et al.48 N/A Bdnf-forward: AGGTCTGACGACGACATCACT PrimerBank49–51 PrimerBank ID 114326460c2 Bdnf-reverse: CTTCGTTGGGCCGAACCTT PrimerBank49–51 PrimerBank ID 114326460c2 Software and algorithms Spike2 software (CED Ltd., Micro1401mkII) CED Ltd https://ced.co.uk/products/spkovin ImageJ (Fiji) Software Schneider et al.52 https://imagej.net/software/fiji/ GraphPad Prism 6 GraphPad Software https://www.graphpad.com/features Others EEG and EMG amplifiers A-M SYSTEMS Cat# 1800 Stereotaxic apparatus Harvard apparatus Cat# 75-1828 Video recording GUCEE Cat# HD98 Centrifugal machine Eppendorf Cat# 5720110011 Enhanced chemiluminescence (ECL) Analytik Jena AG Cat# 849-97-0930 Insulated silver wires Coonerwire Cat# AS633 Frozen ball mill Shanghai Jingxin Cat# JXFSTPRP Vertical Electrophoresis Cell Bio-Rad Cat# 1658001FC PVDF membrane Millipore Cat# IPVH00010 LightCycler 480 Real-Time PCR System Roche Cat# 05015243001 Cryostat Leica CM3050 VT 1200S vibratome Leica Cat# 14048142066 MultiClamp 700B amplifier Axon Instruments Cat# 1-CV-7B Digidata 1440A Axon Instruments Cat# 1-2500-0173 D FN1 microscope Nikon FN1 Borosilicate glass capillaries wpi-europe Cat# B100-20-10 Horizontal glass electrode puller Sutter Cat# P-97 Stimulation electrode FHC Cat# 55-00-13 Confocal microscope Nikon A1 Slide Scanner Olympus VS200
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